تولید و شناسایی نخ نانولیفی دارای لیگنین و امکان‌سنجی استفاده از آن به‌عنوان نخ بخیه دارورسان

نوع مقاله : پژوهشی

نویسندگان

اصفهان، دانشگاه صنعتی اصفهان، دانشکده مهندسی نساجی، صندوق پستی 8415683111

چکیده

فرضیه‌: نخ‌های نانوالیاف تولیدشده از رشته‌های تابیده پلیمرهای الکتروریسی‌شده مانند نایلون، خواص مکانیکی قابل قبولی را به‌عنوان بخیه نشان می‌دهند. استفاده از زیست‌پلیمرهایی مانند لیگنین با داشتن خواص جالبی مانند زیست‌سازگاری، ضداکسندگی، ضدباکتریایی و قابلیت دارورسانی در ساختار نخ‌های نانولیفی می‌توانند خواص نخ بخیه را بهبود بخشند. نخ‌های نانولیفی دارای لیگنین می‌توانند در صورت داشتن استحکام قابل قبول، به‌عنوان نخ بخیه دارورسانی زیست‌سازگار استفاده شوند.
روش‌ها: نخ نانولیفی نایلون و نخ نانولیفی هیبریدی نایلون-لیگنین-پلی(اتیلن اکسید) (N/L/P) و شکل بارگذاری‌شده دارویی آن با روش الکتروریسی تولید شدند. شرایط الکتروریسی بهینه شد و خواص مکانیکی نخ تولیدشده با نخ نانولیفی نایلون مقایسه شد. استحکام نخ هیبریدی گره‌دار (گره مربعی و گره جراحی) با سه سطح پیچش متفاوت بررسی و مقایسه شد. وجود لیگنین و دارو در نخ نانولیفی نایلون N/L/P با طیف‌سنجی FTIR اثبات شد. شکل‌شناسی نخ تولیدشده، سازوکار گسیختگی و تخریب آن با میکروسکوپی FE-SEM بررسی شد. سرعت تخریب نخ در محیط بافر فسفات مطالعه شد. خواص دارورسانی و سازوکارهای رهایش دارو نیز با بارگذاری دارو در نخ نانولیفی N/L/P ارزیابی شد. 
یافته‌ها: مقایسه آماری N/L/P و نخ نانولیفی نایلون نشان داد، تفاوت معنی‌داری در استحکام آن‌ها وجود ندارد. آزمایش گسیختگی نشان داد،‌ نانونخ هیبریدی در مقایسه با گسیختگی تک‌مرحله‌ای نخ نانولیفی نایلون، دارای گسیختگی تک‌مرحله‌ای است. نخ هیبریدی درصد تخریب %46.13 را در 60min نشان داد، در حالی که رهایش دارو از نخ دارای دارو حدود % 97 پس از 4h بود. نتایج نشان داد، نخ نانولیفی N/L/P می‌تواند به‌عنوان نخ بخیه دارورسان با استحکام مکانیکی خوب و در رهایش کوتاه‌مدت دارو به‌کار گرفته شود.

کلیدواژه‌ها

عنوان مقاله [English]

Production and Characterization of Nanofibrous Yarn Containing Lignin and Feasibility Study of Its Use as a Drug Eluting Suture

نویسندگان [English]

  • Saeedeh sadat mirdamadi
  • seyed abdolkarim Hosseini
  • Afsaneh Valipouri
  • laleh ghasemi-mobarakeh
Department of Textile Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran
چکیده [English]

Hypothesis: Nanofibrous yarns produced from twisted strings of electrospun polymers like nylon showed acceptable mechanical properties as sutures. Using biopolymers such as lignin with interesting properties like biocompatibility, antioxidant, anti-bacterial, and drug delivery ability in the form of nanofibrous yarns could improve suture yarn properties. Nanofibrous yarns containing lignin could be used as biocompatible drug delivery suture yarn when having an acceptable strength.
Methods: Nylon nanofibrous yarn, nylon-lignin-polyethylene oxide (N/L/P) hybrid nanofibrous yarn and its drug-loaded form were produced using the electrospinning method. The electrospinning conditions were optimized and the mechanical properties of the produced yarn were compared to a nanofibrous nylon yarn. The strength of knotted hybrid yarn (square knot and surgeon knot) with three different twist levels was examined and compared with each other. The presence of lignin and drug in N/L/P nanoyarn was proved using FTIR spectroscopy. The morphology of the produced yarn, its rupture mechanism, and degradation are studied by FESEM microscopy. The degradation rate of yarn was examined in a phosphate buffer medium. Drug delivery properties and drug release mechanisms are also investigated by loading a drug in N/L/P nanoyarn.   
Findings: Statistical comparison between N/L/P and nylon nanofibrous yarn showed that there is no significant difference in their strength. The rupture test showed that the hybrid nanoyarn has a two-stage rapture compared to the single-stage rapture of nylon nanoyarn. The hybrid yarn showed a degradation percentage of 46.13% in 60 min, while the drug release from the drug-loaded yarn was about 97% after 4 h. In conclusion, the results showed that the N/L/P nanofibrous yarn can be used as a drug delivery suture with good mechanical strength and a proper drug release profile for a short period of time. 

کلیدواژه‌ها [English]

  • thread
  • nanofibrous yarn
  • lignin
  • electrospinning
  • drug release

مراجع

  1. Ratner B.D., Hoffman A.S., Schoen F.J., and Lemons J.E., Biomaterials Science: An Introduction to Materials in Medicine, San Diego, California, 2004.
  2. Chua R., Lim S., Chee C., Chin S., Kiew L., Sim K., and Tay S., Surgical Site Infection and Development of Antimicrobial Sutures: A Review, Rev. Med. Pharmacol. Sci., 26, 828-845, 2022.
  3. Hosseini Ravandi S., Hassanabadi E., Tavanai H., and Abuzade R., Mechanical Properties and Morphology of Hot Drawn Polyacrylonitrile Nanofibrous Yarn, Appl. Polym. Sci., 124, 5002-5009, 2012.
  4. Haghighat F. and Ravandi S.A.H., Mechanical Properties and In Vitro Degradation of PLGA Suture Manufactured via Electrospinning, Fibers Polym., 15, 71-77, 2014.
  5. Minaei F., Ravandi S.A.H., Hejazi S.M., and Alihosseini F., The Fabrication and Characterization of Casein/PEO Nanofibrous Yarn via Electrospinning, e-Polymers, 19, 154-167, 2019.
  6. Mohammadi H., Alihosseini F., and Hosseini S.A., Improving Physical and Biological Properties of Nylon Monofilament as Suture by Chitosan/Hyaluronic Acid, J. Biol. Macromol., 164, 3394-3402, 2020.
  7. Joseph B., George A., Gopi S., Kalarikkal N., and Thomas S., Polymer Sutures for Simultaneous Wound Healing and Drug Delivery–A Review, J. Pharm. Pharm. Sci., 524, 454-466, 2017.
  8. Huh B.K., Kim B.H., Kim S.-N., Park C.G., Lee S.H., Kim K.R., Heo C.Y., and Choy Y.B., Surgical Suture Braided with a Diclofenac-Loaded Strand of Poly(lactic-co-glycolic acid) for Local, Sustained Pain Mitigation, Sci. Eng. C, 79, 209-215, 2017.
  9. Rothenburger S., Spangler D., Bhende S., and Burkley D., In Vitro Antimicrobial Evaluation of Coated VICRYL* Plus Antibacterial Suture (Coated Polyglactin 910 with Triclosan) Using Zone of Inhibition Assays, Infect., 3, s79-s87, 2002.
  10. Ming X., Nichols M., and Rothenburger S., In Vivo Antibacterial Efficacy of Monocryl Plus Antibacterial Suture (Poliglecaprone 25 with Triclosan), Infect., 8, 209-214, 2007.
  11. Richard A.S. and Verma R.S., Bioactive Nano Yarns as Surgical Sutures for Wound Healing, Sci. Eng., C, 128, 112334, 2021.
  12. Rouhollahi F., Hosseini S.A., Alihosseini F., Allafchian A., and Haghighat F., Investigation on the Biodegradability and Antibacterial Properties of Nanohybrid Suture Based on Silver Incorporated PGA-PLGA Nanofibers, Fibers Polym., 19, 2056-2065, 2018.
  13. Madheswaran D., Sivan M., Valtera J., Kostakova E.K., Egghe T., Asadian M., Novotny V., Nguyen N.H., Sevcu A., and Morent R., Composite Yarns with Antibacterial Nanofibrous Sheaths Produced by Collectorless Alternating-Current Electrospinning for Suture Applications, Appl. Polym. Sci., 139, 51851, 2022.
  14. Yadong Z., Sugarcane Biomass as а Potential Carrier for Drug Delivery System, NTUU Series, 50-56, 2021.
  15. Li J., Isolation of Lignin from Wood, BSc Thesis, Saimaa University of Applied Sciences, Imatra, 2011.
  16. Pourmahdi M., Abdollahi M., and Nasiri A., Synthesis and Characterization of Native Lignin/Acrylamide-Based Copolymers Obtained by Graft Radical Polymerization, J. Polym. Sci. Technol. (Persian), 30, 501-516, 2018.
  17. Figueiredo P., Lintinen K., Hirvonen J.T., Kostiainen M.A., and Santos H.A., Properties and Chemical Modifications of Lignin: Towards Lignin-Based Nanomaterials for Biomedical Applications, Mater. Sci., 93, 233-269, 2018.
  18. Abdelaziz O.Y., Brink D.P., Prothmann J., Ravi K., Sun M., García-Hidalgo J., Sandahl M., Hulteberg C.P., Turner C., and Lidén G., Biological Valorization of Low Molecular Weight Llignin, Adv., 34, 1318-1346, 2016.
  19. Kubo S. and Kadla J., Lignin-Based Carbon Fibers: Effect of Synthetic Polymer Blending on Fiber Properties, Polym. Environ., 13, 97-105, 2005.
  20. Poursorkhabi V., Mohanty A.K., and Misra M., Electrospinning of Aqueous Lignin/Poly(ethylene oxide) Complexes, Appl. Polym. Sci, 132, 2015.
  21. Gupta A.K., Mohanty S., and Nayak S.K., Preparation and Characterization of Lignin Nanofibre by Electrospinnig Technique, J. Sci. Eng. Appl. Sci., 1, 184-190, 2015.
  22. Wang J., Tian L., Luo B., Ramakrishna S., Kai D., Loh X.J., Yang I.H., Deen G.R., and Mo X., Engineering PCL/Lignin Nanofibers as an Antioxidant Scaffold for the Growth of Neuron and Schwann Cell, Colloids Surf. B, 169, 356-365, 2018.
  23. Kai D., Jiang S., Low Z.W., and Loh X.J., Engineering Highly Stretchable Lignin-Based Electrospun Nanofibers for Potential Biomedical Applications, Mater. Chem. B, 3, 6194-6204, 2015.
  24. Reesi F., Minaiyan M., and Taheri A., A Novel Lignin-Based Nanofibrous Dressing Containing Arginine for Wound-Healing Applications, Drug Deliv. Transl. Res., 8, 111-122, 2018.
  25. Zeighampour F., Alihosseini F., Morshed M., and Rahimi A.A., Comparison of Prolonged Antibacterial Activity and Release Profile of Propolis-Incorporated PVA Nanofibrous Mat, Microfibrous Mat, and Film, Appl. Polym. Sci., 135, 45794-n/a, 2018.
  26. Zamani M., Morshed M., Varshosaz J., and Jannesari M., Controlled Release of Metronidazole Benzoate from Poly- ε-Caprolactone Electrospun Nanofibers for Periodontal Diseases, J. Pharm. Biopharm., 75, 179-185, 2010.
  27. Jannesari M., Varshosaz J., Morshed, M., Zamani, M., Composite Poly(vinyl alcohol)/Poly(vinyl acetate) Electrospun Nanofibrous Mats as a Novel Wound Dressing Matrix for Controlled Release of Drugs, J. Nanomed., 6, 993-1003, 2011.
  28. Li X., Yao C., Sun F., Song T., Li Y., and Pu Y., Conjugate Electrospinning of Continuous Nanofiber Yarn of Poly(L-lactide)/Nanotricalcium Phosphate Nanocomposite, Appl. Polym. Sci., 107, 3756-3764, 2008.
  29. Formic Acid., Material Safety Data Sheet, Scholarchemistry.com, Available in 20 June 2022.
  30. Dimethylformamide, Material Safety Data Sheet, americanbio.com, Available in 20 June 2022.
  31. Hosseini S.A., Lin L.-T., and Ko F., Investigation of the Mechanical Properties of Lignin Nanofibrous Structures Using Statistical Modeling, Res. J., 88, 1943-1953, 2018.
  32. Sanatgar R.H., Borhani S., Ravandi S.A.H., and Gharehaghaji A.A., The Influence of Solvent Type and Polymer Concentration on the Physical Properties of Solid State Polymerized PA66 Nanofiber Yarn, Appl. Polym. Sci., 126, 1112-1120, 2012.
  33. Najafi S., Gharehaghaji A., and Etrati S., Fabrication and Characterization of Elastic Hollow Nanofibrous PU Yarn, Des., 99, 328-334, 2016.
  34. Mohammadi H., Fabrication of Nanofibrous Yarn Using Chitosan/Hyaluronic Acid Polyelectrolyte System for Eye Surgery Sutures, PhD Thesis, School of Textile Engineering, Isfahan University of Technology, 2020.
  35. Yang Z., Shen C., Zou Y., Wu D., Zhang H., and Chen K., Application of Solution Blow Spinning for Rapid Fabrication of Gelatin/Nylon 66 Nanofibrous Film, Foods, 10, 2339, 2021.
  36. Liu H. and Leonas K.K., Weight Loss and Morphology Changes of Electrospun Poly(ɛ-caprolactone) Yarns During In Vitro Degradation, Fibers Polym., 11, 1024-1031, 2010.
  37. Hasan M., Hossain M., Sultana A., Shoeb M., and Nahar N., Evaluation of Diclofenac by UV-Vis Spectrophotometer in Some Locally Produced Tablets, Dhaka Univ. J. Sci., 65, 163-165, 2017.
  38. Galkina O., Ivanov V., Agafonov A., Seisenbaeva G., and Kessler V., Cellulose Nanofiber-Titania Nanocomposites as Potential Drug Delivery Systems for Dermal Applications, Mater. Chem. B, 3, 1688-1698, 2015.
  39. Ritger P.L. and Peppas N.A., A Simple Equation for Description of Solute Release I. Fickian and Non-Fickian Release from Non-Swellable Devices in the Form of Slabs, Spheres, Cylinders or Discs, Control Release, 5, 23-36, 1987.
  40. Barzegar J.M. and Valizadeh H., Mohammadi G., and Adibkia K., Analytical Review of Drug Dissolution and Release Kinetic Models, Sci., 14, 91-207, 2009.
  41. Paarakh M.P., Jose P.A., Setty C., and Christoper G.P., Release Kinetics-Concepts and Applications, J. Pharm. Res. Technol., 8, 12-20, 2018.

 

مجله علوم و تکنولوژی پلیمر
دوره 35، شماره 2 - شماره پیاپی 178
خرداد و تیر 1401
صفحه 111-125

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