تهیه پلیمر-کمپلکس جدید با نگهدارنده آهن و بررسی خواص ضدسرطانی آن

نوع مقاله : پژوهشی

نویسندگان

گروه شیمی آلی، دانشکده شیمی، دانشگاه لرستان، خرم‌آباد، لرستان

چکیده

فرضیه‌: پلی(1-(2-((3-آمینو-2-هیدروکسی‌پروپیل)آمینو)اتیل)-'1-اتیل-[4،'4-بی‌پیریدین]-1،'1-دی‌ایوم) ((Poly(AHAEBD) به‌دلیل داشتن گروه‌های عاملی متناوب هیدرکسی و آمین نوع دوم، بسیار مستعد واکنش‌های تشکیل کمپلکس است. در کار حاضر فلز آهن (ΙΙΙ) به‌دلیل نقش مؤثر در فعالیت‌های متابولیکی بدن، برای کمپلکس با (Poly(AHAEBD انتخاب و آثار ضدسرطانی آن بر رده یاخته‌ای سرطان پستان انسانی (MCF-7) بررسی شد.
روش‌ها: ابتدا، ترکیب 1،'1-بیس(2-آمینواتیل)-[4،'4-بی‌پیریدین]-1،'1-دی‌ایوم (BABD) از واکنش 4،'4-بی‌پیریدین و 2-آمینواتیل برمید تهیه شد. سپس پلیمر ((Poly(AHAEBD) از واکنش BABD و اپی‌کلروهیدرین سنتز و کمپلکس آن با آهن (ΙΙΙ) و(Fe(poly(AHAEBD)2].Na3]) ساخته شد. ساختار ترکیبات سنتزشده با استفاده از طیف‌‌نمایی زیرقرمز تبدیل فوریه (FTIR)، طیف‌نمایی رزونانس مغناطیسی هسته (1H NMR)، میکروسکوپ الکترونی پویشی (FE-SEM)، طیف‌سنجی پاشنده انرژی پرتو X و سوانگاری ژل‌تراوشی (GPC) تأیید شد. اثر ترکیبات (Fe(poly(AHAEBD)2].Na3] و(Poly(AHAEBD و سیس‌پلاتین به‌عنوان مرجع، روی رده یاخته‌ای MCF-7  با آزمون MTT بررسی شد. 
یافته‌ها: مقدار IC50 پلیمر کمپلکس Fe(poly(AHAEBD)2].Na3] (0.53µg/mL )، سیس‌پلاتین (2.68µg/mL ) و (Poly(AHAEBD به‌دست آمد که نشان‌دهنده عملکرد بهتر این پلیمر کمپلکس نسبت به سیس‌پلاتین و نیز اثر تشکیل کمپلکس با آهن در افزایش مقدار سمیت نسبت به ترکیب (Poly(AHAEBD است. اثر پلیمر سنتزشده بر رده یاخته سرطانی MCF-7 از یک طرف و اثر افزایش مقدار آهن در توقف رشد یاخته‌های سرطانی، از طرف دیگر باعث عملکرد مؤثر این پلیمر کمپلکس در توقف رشد یاخته‌های سرطانی در آزمون‌های برون‌تنی شده است. براساس نتایج، از این پلیمر کمپلکس می‌توان به‌عنوان انتخابی بالقوه در بررسی‌های بیشتر برای داروی ضدسرطان استفاده کرد.

کلیدواژه‌ها

عنوان مقاله [English]

Preparation of a Novel Polymer-Complex Supported Iron (ΙΙΙ) and Study of Its Anti-Cancer Properties

نویسندگان [English]

  • Siavash Hasanvandi
  • ebrahim mehdipour
  • parvaneh shafieyoon
Department of Organic Chemistry, Faculty of Chemistry, Lorestan University, Khorramabad, Lorestan
چکیده [English]

Hypothesis: Due to the alternating hydroxyl and secondary amine groups, the polymer poly(1-(2-((3-amino-2-hydroxypropyl)amino)ethyl)-1'-ethyl-[4,4'-bipyridine]-1,1'-diium) (poly(AHAEBD)), is highly prone to complex formation. In this case, iron (III) was selected for complexation with poly(AHAEBD) due to its effective role in metabolic activities in the body then, anticancer effects of the synthesized compounds were evaluated on human breast cancer cells (MCF-7). 
Methods: 1,1'-Bis(2-aminoethyl)-[4,4'-bipyridine]-1,1'-diium (BABD) was prepared from the reaction of 4,4'-bipyridine and 2-aminoethyl. The polymer, (poly(AHAEBD)), was synthesized from the reaction of BABD and epichlorohydrin, and then complexed with iron (III) to prepare ([Fe(poly(AHAEBD)2].Na3). The structure of the synthesized compounds was confirmed using Fourier transfer infrared spectroscopy (FTIR), proton nuclear magnetic resonance (1H NMR) spectroscopy, field emission scanning electron microscopy (FE-SEM), energy-dispersive spectroscopy (EDS), and gel permeation chromatography (GPC). The effects of [Fe(poly(AHAEBD)2].Na3, poly(AHAEBD), and cisplatin as a reference were evaluated on MCF-7 cell line using the MTT assay. 
Findings: The IC50 values for the polymer complex [Fe(poly(AHAEBD)2].Na3 (0.53 µg/mL), cisplatin (2.68 µg/mL), and poly(AHAEBD) (1.14 µg/mL) were obtained, indicating the superior performance of this polymer complex in inhibiting cancer cell growth compared to cisplatin as well as displaying the effect of complex formation with iron in increase the cytotoxicity compared to poly(AHAEBD). The impact of the synthesized polymer on the MCF-7 cancer cell line, on one hand, as well as the effect of increased iron levels in stopping the growth of cancer cells, and on the other hand, it has resulted in the effective performance of this complex polymer in stopping the growth of cancer cells in in vitro tests. Based on the results obtained, this polymer complex can be considered as a potential candidate for further investigation as an anticancer drug.

کلیدواژه‌ها [English]

  • Bipyridine derivative
  • Iron complex polymer
  • Cancer cell line
  • Anti-cancer drug
  • Supporter

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مجله علوم و تکنولوژی پلیمر
دوره 36، شماره 2 - شماره پیاپی 184
خرداد و تیر 1402
صفحه 167-176

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