سنتز و کاربرد دانه‌های هیدروژل زیست‌نانوکامپوزیت نقطه کوانتومی گرافن مغناطیسی-کیتوسان برای سامانه دارورسانی

نوع مقاله : پژوهشی

نویسندگان

تبریز، دانشگاه تبریز، دانشکده شیمی، گروه شیمی کاربردی،کد پستی 5166616471

چکیده

فرضیه: در سال‌های اخیر دانشمندان و پژوهشگران به دنبال مواد جدید و پیشرفته‌ای برای استفاده در زمینه‌های مختلف از جمله دارورسانی و زیست‌فناوری بوده‌اند. یکی از مواد جالبی که در این زمینه مورد توجه قرار گرفته است، دانه‌های هیدروژل زیست‌نانوکامپوزیت بر پایه کیتوسان است. دانه‌های هیدروژل زیست‌نانوکامپوزیت بر پایه کیتوسان به‌دلیل داشتن ویژگی‌های ذاتی مانند زیست‌سازگاری عالی، تورم زیاد و ظرفیت‌های ذخیره‌سازی زیاد، توجه‌ها را به‌عنوان حامل در سامانه‌های دارورسانی جلب کرده‌اند.
روش‌ها: در این پژوهش، ابتدا نقطه کوانتومی گرافن (GQD) به روش تجزیه گرمایی تهیه شد و پس از آن با استفاده از نانوذرات آهن خاصیت مغناطیسی پیدا کرد (MGQD)، در ادامه با هیدروژل پلیمری کیتوسان پوشش یافت (CS-MGQD) و سپس داروی متوترکسات (MTX) در آن بارگذاری شد (MTX/CS-MGQD). این ترکیب  با خواص منحصربه‌فرد هیدروژل کیتوسان، خواص مغناطیسی نقاط کوانتومی گرافن و قابلیت تنظیم رهایش دارو توانست نقطه عطف مهمی در زمینه پژوهش‌های دارورسانی و سازگاری داروها با محیط‌زیست ایجاد کند. سنتز و تشکیل ترکیب مدنظر به‌کمک آزمون‌های مختلف از جمله FTIR برای شناسایی گروه‌های عاملی، XRD برای شناسایی ساختار بلوری و SEM برای بررسی شکل‌شناسی نمونه‌، تأیید شد.
یافته‌ها: حدود ۸۴ و %۶۴ از MTX به‌ترتیب در دانه‌های هیدروژل CS و CS-MGQD بارگذاری شد. بر اساس نتایج رفتار تورم و رفتار رهایش دارو، دانه‌های هیدروژل تورم وابسته به pH و رهایش MTX را نشان دادند. افزون بر این، CS-MGQD حساسیت به رفتار تورم و رهایش دارو نشان داد که دارای ثبات مطلوب و رهایش دارو قابل ‌کنترل در محیط اسیدی است. همچنین، مدل سینتیکی Weibull بهترین مدل برای رهایش MTX (متوترکسات) از CS-MGQD در pH برابر 5 بود. این یافته‌ها نشان می‌دهد، دانه‌های هیدروژل زیست‌نانوکامپوزیت مغناطیسی تهیه‌شده قابلیت خوبی برای دارورسانی قابل کاشت حساس به pH در درمان بافت سرطانی دارند.

کلیدواژه‌ها

عنوان مقاله [English]

Magnetic Graphene Quantum Dot/Chitosan Bionanocomposite Hydrogel Beads for Drug Delivery System: Synthesis and Application

نویسندگان [English]

  • Hossein Poursadegh
  • Mehdi Barzegarzadeh
  • Mohammad Sadegh Aminifazl
Advanced Polymer Material Research Laboratory, Department of Applied Chemistry, Faculty of Chemistry, University of Tabriz, Postal Code 5166616471, Tabriz, Iran
چکیده [English]

Hypothesis: In recent years, scientists and researchers have been looking for new and advanced materials for use in various fields, including drug delivery and biotechnology. One of the attractive  materials that has been considered in this field is chitosan-based bionanocomposite hydrogel beads. Chitosan-based bionanocomposite hydrogel beads have attracted attention as carriers in drug delivery systems due to their inherent properties such as excellent biocompatibility, high swelling, and high storage capacities.
Methods: First graphene quantum dots (GQDs) were prepared by the pyrolysis method, and then their magnetic properties were obtained using iron nanoparticles (MGQD). Next they were coated with chitosan hydrogel (CS-MGQD) and finally loaded with methotrexate (MTX/CS-MGQD). This unique combination of the properties of chitosan hydrogel, the magnetic properties of graphene quantum dots, and the ability to adjust drug release has been able to create an important milestone in the field of drug delivery research and the compatibility of drugs with the biological environment. Through various analyses, including FTIR to analyze the spectra of the functional groups, XRD to identify the crystal structure, and SEM to examine the morphology of the samples, the success of the synthesis and formation of the desired compound was confirmed.
Findings: The fabricated CS and CS-MGQD hydrogel beads were loaded with about 84% and 64% MTX, respectively. The results of the swelling behavior and drug release behavior showed that the hydrogel beads experience pH-dependent swelling and release of MTX. In addition, investigating the effect of MGQD concentration on swelling behavior and drug release showed that CS-MGQD has favorable stability and controllable drug release in an acid environment. Also, the Weibull kinetic model was found to be the best-fitting model for the release of MTX from CS-MGQD at pH 5. These findings suggest that the prepared magnetic bionanocomposite hydrogel beads have a good potential for pH-sensitive implantable drug delivery in the treatment of cancerous tissue.

کلیدواژه‌ها [English]

  • Bionanocomposite
  • Hydrogel beads
  • Chitosan
  • Magnetic Graphene quantum dot
  • Drug delivery

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مجله علوم و تکنولوژی پلیمر
دوره 36، شماره 4 - شماره پیاپی 186
مهر و آبان 1402
صفحه 409-420

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